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AB10626 Sigma-Aldrich

Anti-IL-1 β Antibody

clone, Chemicon®, from rabbit

  •  eCl@ss 32160702

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Properties

Related Categories Alphabetical Index, Antibodies, IH-IM, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   pig, swine
shipped in   dry ice
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_000576.2
UniProt accession no.   P01584

Description

General description

This antibody recognizes natural and recombinant swine IL-1 . either a 269 AA or a 271 AA (17 kDa) protein with no disulfide bonds. There are two distinct molecular forms of IL-1 (IL-1 and IL-1 ) derived from two different genes.

Specificity

Other species were not tested.

Recognizes IL-1 Beta.

Immunogen

Recombinant swine IL-1β.

Application

Anti-IL-1 β Antibody is an antibody against IL-1 β for use in WB.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

This antibody is suitable for use in Western blot analysis at 0.5 - 2.0 ug/mL.
This antibody is non-neutralizing when tested by proliferation assay.

Physical form

Format: Purified

Protein G Purified

Rabbit polyclonal antibodies in phosphate buffered saline, pH 7.2.

Storage and Stability

Store at -20°C in undiluted aliquots for 1 year from date of shipment.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Linkage

Replaces: 04-462; MABF22

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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