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AB1211 Sigma-Aldrich

Anti-β Galactosidase Antibody

Anti-β Galactosidase Antibody is an antibody against β Galactosidase for use in ELISA, Immunohistochemistry, Immunoprecipitation, Western Blotting.

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Properties

Related Categories Antibodies, Primary Antibodies More...
trade name   Chemicon
brand family   Chemicon
format   Purified
control   Proteins tagged with beta galactosidase
application(s)   ELISA
  Immunohistochemistry
  Immunoprecipitation
  Western Blotting
clone   Polyclonal Antibody
purification method   Ammonium sulfate precipitation and DEAE-cellulose chromatography
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
host   Rabbit
species reactivity   Vertebrates, E. coli
NCBI accession no.   NM_000404.2, NM_001079811.1
UniProt accession no.   P16278
gene symbol   EBP(10682)
  GLB1(2720)
  ELNR1
  LACTASE
  S-GAL
material size   100 µL
shipped in   dry ice
storage conditions   Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Description

Physical form

Immunoglobulin Purified by delipidation, salt fractionation and ion-exchange chromatography. Liquid in 0.02M Potassium Phosphate buffer, 0.15M Sodium Chloride, with 0.01% sodium azide.

Application

ELISA: 1:10,000-1:50,000 when assayed against 1 μg of Beta Galactosidase in a standard sandwich ELISA.

Immunoblotting: 1:2000-1:10,000 on beta-galactosidase fusion proteins.

Immunohistochemistry: 1:1,000-1:5000 on tissue fixed with 4% paraformaldehyde.

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Specificity

Recognizes Beta-Galactosidase (E. coli). Monospecific by IEP. Some cross reactivity with Beta Galactosidase from other species may occur.

Immunogen

Beta-Galactosidase (E. coli).

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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