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  • AB1224 - Anti-Myeloperoxidase Antibody, human white cell

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AB1224 Sigma-Aldrich

Anti-Myeloperoxidase Antibody, human white cell

serum, clone, Chemicon®

Synonym: MPO

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Antibodies for Cell Biology, Antibodies to Chemokines, Antibodies to Cytokines and Growth Factors, Primary Antibodies More...
Quality Level   100
biological source   rabbit
antibody form   serum
clone   polyclonal
species reactivity   human
mfr. no.   Chemicon®
application(s)   ELISA: suitable
  western blot: suitable
NCBI accession no.   NM_000250.1
UniProt accession no.   P05164
shipped in   wet ice

Description

Specificity

Myeloperoxidase (Human White Cells). Monospecific by IEP. Some cross reactivity with Myeloperoxidase from other tissues and species may occur.

Myeloperoxidase (MPO) represents an early appearing and highly reliable intracellular myeloid lineage marker molecule. MPO protein can be detected in a subset of human hematopoietic bone marrow progenitor cells and in granulomonopoietic (GM) cells. However, other myeloid-related cell types such as epidermal Langerhans-type dendritic cells (LC) lack MPO. Therefore, human myeloid progenitors might be subdivided based on MPO protein expression into functional subsets.

Immunogen

Epitope: human white cell

Myeloperoxidase (Human White Cells)

Application

Anti-Myeloperoxidase Antibody, human white cell is an antibody against Myeloperoxidase for use in ELISA & WB.

Immunoblotting: antibody recognizes alpha and beta subunits of MPO. Reduced and denatured extracts of purified MPO produce blots produce 55-60kDa alpha subunits and 12-14kDa beta subunit bands140 Additional bands may be present due to cross reactivity with other myeloperoxidases.

ELISA:

Optimal working dilutions must be determined by end user.

Physical form

Monospecific antiserum purified by delipidation and defibrination. Lyophilized from 0.02 M Potassium Phosphate buffer, 0.15 M Sodium Chloride, pH 7.2, 0.01% Sodium Azide. Reconstitute with 2.0 mL of sterile distilled water.

Storage and Stability

Store unopened vial at 2-8 ºC. Maintain frozen at -20ºC in undiluted aliquots for up to one year after reconstitution. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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