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  • AB143 - Anti-Choline Acetyltransferase (ChAT) Antibody

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AB143 Sigma-Aldrich

Anti-Choline Acetyltransferase (ChAT) Antibody

Synonym: Choline acetylase, acetyl CoA:choline O-acetyltransferase, choline acetyltransferase

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, CE-CH, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable (paraffin)
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   feline, bat, monkey, monkey, human, mouse, feline, rat, mouse, rat, bat, human
shipped in   dry ice
antibody form   serum
antibody product type   primary antibodies
Quality Level   100
mfr. no.   Chemicon®
NCBI accession no.   NP_065574
UniProt accession no.   P28329

Description

General description

Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.

Specificity

Cholinergic neurons in brain and central nervous systems.

Reactivity with other species has not been confirmed with IHC.

Application

Immunoprecipitation:
1:200 dilution of a previous lot immunoprecipitated 68 kDa active ChAT from brain and placenta. Also precipitates a 27 kDa protein that is co-expressed with ChAT (but lacking ChAT activity) in some cholinergic neurons of the central nervous system of Humans and possibly other mammals.

Optimal working dilutions and protocls must be determined by end user.

Physical form

Rabbit polyclonal antisera, liquid, no preservatives.

Quality

Routinely evaluated by immunohistochemistry (paraffin) on normal cerebral cortex tissue.

Immunohistochemistry (Paraffin) Analysis:
ChAT (cat. # AB143) staining on normal cerebral cortex. Tissue treated with no antigen retrieval. The antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern on the cell body, dendrites and axons of neurons (brown).

Analysis Note

Control
Cerebral cortex tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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