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AB144P Sigma-Aldrich

Anti-Choline Acetyltransferase Antibody

Anti-Choline Acetyltransferase Antibody detects level of ChAT and has been published and validated for use in IH(P), IC, IH and WB.

Synonym: Choline acetylase, acetyl CoA:choline O-acetyltransferase, choline acetyltransferase, ChAT

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, CE-CH, Primary Antibodies
clone   polyclonal
biological source   goat
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   guinea pig, mouse, opossum, zebrafish (THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).), human, zebrafish, avian, mouse, rat, chicken, monkey, human
shipped in   dry ice
antibody product type   primary antibodies
Quality Level   100
purified by   affinity chromatography
mfr. no.   Chemicon®
NCBI accession no.   NM_020549.3
UniProt accession no.   P28329

Description

General description

Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker.

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Specificity

Choline acetyltransferase found in cholinergic neurons in brain and spinal cord.

Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.

Immunogen

Human placental enzyme.

Application

Immunohistochemistry:
A previous lot of this antibody was used at 1:100.

Optimal working dilutions and protocols must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Target description

70/74 kDa

Physical form

Affinity Purfied

Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
For short term storage (3-4 weeks) maintain at 2-8°C.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.

Analysis Note

Control
Human placenta lysates, rat forebrain/rostral hypothalamus.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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