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  • AB144P - Anti-Choline Acetyltransferase Antibody

AB144P Sigma-Aldrich

Anti-Choline Acetyltransferase Antibody

clone, Chemicon®, from goat

Synonym: Choline acetylase, acetyl CoA:choline O-acetyltransferase, choline acetyltransferase, ChAT

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, CE-CH, Primary Antibodies
clone   polyclonal
biological source   goat
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   guinea pig, mouse, opossum, zebrafish (THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).), human, zebrafish, avian, mouse, rat, chicken, monkey, human
shipped in   dry ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
mfr. no.   Chemicon®
NCBI accession no.   NM_020549.3
UniProt accession no.   P28329


General description

Choline acetyltransferase (ChAT) has a molecular weight of about 68 kDa. It is synthesized in the parakaryon and is transported to the axon terminals. ChAT belongs to the choline/carnitine acyltransferase family.


Choline acetyltransferase found in cholinergic neurons in brain and spinal cord.

Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.


Human placental enzyme.


Anti-Choline Acetyltransferase Antibody has been used in immunohistochemical staining.

Target description

70/74 kDa

Physical form

Affinity Purfied

Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
For short term storage (3-4 weeks) maintain at 2-8°C.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Choline acetyltransferase (CHAT) is involved in the synthesis of acetylcholine in neurons. It catalyzes the transfer of an acetyl group between acetyl-coenzyme A and choline. Mutations in the gene encoding this protein have been associated with congenital neuromuscular disorders.


Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.

Analysis Note

Human placenta lysates, rat forebrain/rostral hypothalamus.

Safety & Documentation

Safety Information

WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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