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  • AB1520 - Anti-Glutamate Transporter Antibody, neuronal

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AB1520 Sigma-Aldrich

Anti-Glutamate Transporter Antibody, neuronal

serum, Chemicon®

Synonym: EAAC1, EAAT3

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, GD-GL, Primary Antibodies
clone   polyclonal
biological source   goat
application(s)   immunohistochemistry: suitable
species reactivity   guinea pig, rat, mouse
shipped in   dry ice
antibody form   serum
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
suitability   not suitable for Western blot
NCBI accession no.   NM_004170.4
UniProt accession no.   P43005
Gene Information   human ... SLC1A1(6505)

Description

Specificity

Neuronal Glutamate Transporter (EAAC1; also called EAAT3). The antibody has been tested on central nervous system tissue. The staining pattern for the cell bodies obtained with AB1520 corresponds to the pattern described using in situ hybridization with probes to rEAAC1 mRNA. The immunoreactivity corresponds to what has been described for glutamatergic neuronal systems. Preabsorption of the antiserum with the immunogen peptide completely abolishes the immunostaining.

Immunogen

Synthetic peptide from the carboxy-terminus of the cloned rat EAAC1.

Application

Anti-Glutamate Transporter Antibody, neuronal detects level of Glutamate Transporter & has been published & validated for use in IH.

Immunohistochemistry: 1:4,000-8,000 using a cyanine-conjugated secondary antibody. Enzymatic methods of detections can be used, however antibody dilution must be increased to 1:10,000-1:30,000. Lightly fixed, 4% PFA material is recommended. The use of triton X-100 in the block only is recommended.

This antibody is not recommended for use in Western blot.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Physical form

Goat antiserum. Liquid, no preservatives.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to one year.

Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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