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AB1528 Sigma-Aldrich

Anti-Nerve Growth Factor-β Antibody

Detect Nerve Growth Factor-β using this Anti-Nerve Growth Factor-β Antibody validated for use in ELISA, WB, IH.

Synonym: NGFbeta

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, NE-NI, Primary Antibodies
clone   polyclonal
biological source   sheep
application(s)   ELISA: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   chicken, mouse, rat, human
should not react with   bovine
shipped in   dry ice
antibody form   serum
antibody product type   primary antibodies
Quality Level   100
mfr. no.   Chemicon®
NCBI accession no.   NM_002506.2
UniProt accession no.   P01138
Gene Information   human ... NGF(4803)

Description

Specificity

Nerve growth factor-beta (NGF). Less than 1% cross-reactivity against recombinant human NT4 and BDNF and approximately 2% for NT3 by ELISA.

Immunogen

Mouse nerve growth factor-beta purified from salivary glands.

Application

Immunohistochemistry at 1:500-1:5,000 (see suggested protocol).

Immunoblotting at 1:500-1:5,000.

ELISA at 1:500-1:5,000.

Inhibition of biological activity in vitro at 1:10-1:50.
BIOLOGICAL ACTIVITY: Neutralizes NGF, but not other neurotrophins.

Use neat for in vivo animal studies (5-10 μL/g body weight).

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurochemistry & Neurotrophins

Physical form

Whole sheep antisera. Lyophilized. Reconstitute with 250 μL of sterile distilled water.

Storage and Stability

Maintain at -20°C to -70°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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