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AB1529 Sigma-Aldrich

Anti-Nitric Oxide Synthase I Antibody

Synonym: NOS I, bNOS, nNOS

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, NE-NI, Primary Antibodies
clone   polyclonal
biological source   sheep
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   rabbit, rat, guinea pig
shipped in   wet ice
antibody form   serum
antibody product type   primary antibodies
Quality Level   100
mfr. no.   Chemicon®
NCBI accession no.   NM_000620.1
UniProt accession no.   P29475

Description

Specificity

bNOS (neuronal). Not yet tested for cross-reactivity to iNOS or eNOS. See reference below.

Immunogen

A 21 amino acid peptide (1409-1429) of the neuronal form of NOS from rat cerebellum, coupled to KLH. The sequence is H-RSESIAFIEESKKDADEVFSS-NH2.

Application

Immunohistochemistry: 1:500-1,000

Immunoblotting

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Oxidative Stress

Physical form

Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue.

Storage and Stability

Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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