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AB152 Sigma-Aldrich

Anti-Tyrosine Hydroxylase Antibody

clone, Chemicon®, from rabbit

Synonym: Tyrosine 3-hydroxylase, Tyrosine 3-monooxygenase, tyrosine hydroxylase, TH

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, TS-TZ
clone   polyclonal
biological source   rabbit
application(s)   ELISA: suitable
  immunofluorescence: suitable
  immunohistochemistry: suitable (paraffin)
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse, mouse, rat, mollusk, ferret, feline, squid, human, ferret, rat, feline, Aplysia (marine mollusc), Drosophila
shipped in   dry ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
mfr. no.   Chemicon®
NCBI accession no.   NM_012740.3
UniProt accession no.   P04177

Description

General description

Tyrosine hydroxylase (also know as Tyrosine 3-hydroxylase and Tyrosine 3-monooxygenase) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Specificity

It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.

Tyrosine hydroxylase. By western blot, AB152 selective labels a single band at approximately 62kDa (reduced) corresponding to Tyrosine Hydroxylase. It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.

Immunogen

Denatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).

Application

Anti-Tyrosine Hydroxylase Antibody detects level of TH and has been published and validated for use in ELISA, IF, IH, IH(P), IP and WB.

Immunohistochemistry:
A previous lot of this antibody was uesd at 1:100-1:1,000 dilution, 4°C, for 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.

Immunofluorescence:
A previous lot of this antibody was used at 1:100-1:1,000 dilution.

Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.

ELISA:
A previous lot of this antibody was used in ELISA.

Optimal dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Target description

62 kDa

Physical form

Affinity Purfied

Purified rabbit polyclonal in buffer containing liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl (pH 7.5), 100 ug/mL BSA and 50% glycerol.

Storage and Stability

Maintain frozen at -20ºC to -70ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of PC12 lysates.

Analysis Note

Control
POSITIVE CONTROL: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands.
NEGATATIVE CONTROL: Liver.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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