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AB1543 Sigma-Aldrich

Anti-Synapsin I Antibody

serum, clone, Chemicon®

Synonym: Synapsin-1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SU-SY
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   rat, bovine, human, mouse
shipped in   dry ice
antibody form   serum
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_006950.3
UniProt accession no.   P17600
Gene Information   human ... SYN1(6853)

Description

General description

Synapsin I is a member of the synapsin family. Synapsins are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations of the Synapsin I gene may be associated with X linked disorders with primary neuronal degeneration such as Rett syndrome.

Specificity

Synapsin I (Mr of 80,000 and 77,000). Immunolabeling is blocked by preadsorption of antibody with Synapsin I. Synapsin is located only in brain nerve terminal and is an excellent marker for synapses.

Immunogen

Synapsin I (mixture of Ia & Ib) purified from bovine brain.

Application

Detect Synapsin I using this Anti-Synapsin I Antibody validated for use in IC, IH(P), IP & WB.

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

Western blotting:
1:200 of a previous lot was used for labeled Protein A, 1:1,000 dilution for ECL.

Immunocytochemistry:
1:500-1:2,000 dilution of a previous lot was used.

Immunoprecipitation:
5 μL of a previous lot quantitatively immunoprecipitated all synapsin in 200 μg of rat brain.

Optimal working dilutions must be determined by the end user.

Target description

77 & 80 kDa

Physical form

Rabbit polyclonal antiserum in buffer containing no preservatives.

Unpurified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality

Immunohistochemistry(paraffin): Synapsin representative staining pattern/morphology in rat hippocampal neurons. Tissue was pretreated with Citrate pH 6.0, antigen retrieval. A previous lot of this antibody was diluted to 1:1000, using IHC-Select reagents used with HRP-DAB. Immunoreactivity is seen directly associated with terminal end of neuronal cell body.
IHC-Paraffin Staining With Epitope Retrieval: Rat Hippocampus

Analysis Note

Control
Mouse hippocampal primary neurons, Mouse brain lysates, PC-12 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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