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AB1559 Sigma-Aldrich

Anti-Adenosine A2a Receptor Antibody

This Anti-Adenosine A2a Receptor Antibody is validated for use in ELISA, WB for the detection of Adenosine A2a Receptor.

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   50 µL (AB1559)
antibody form   serum
application(s)   ELISA: suitable
  western blot: suitable
biological source   rabbit
clone   polyclonal
shipped in   dry ice
species reactivity   rat, bovine, canine, human, pig
suitability   not suitable for immunohistochemistry
gene symbol   ADORA2A(135)
trade name   Chemicon
brand family   Chemicon
gene symbol   ADORA2
  RDC8
  hA2aR

Description

Application

Western blot: 1:1,000-1:5,000 (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.

ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG285)/well)

Optimal working dilutions must be determined by end user.

Immunogen

Synthetic 30 amino acid peptide corresponding to amino acids 383-412 of the canine adenosine A2a receptor near the C-terminal intracellular domain (30 amino acids) of the cloned canine A2a receptor. The A2a sequence selected in not present in A1, A2b and A3 receptors.

Physical form

Rabbit antiserum. Liquid containing 0.1% sodium azide.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Making Headway
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
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References

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