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AB1570W Sigma-Aldrich

Anti-GABA Transporter-1 Antibody

clone, Chemicon®, from rabbit

Synonym: GAT-1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, G-GC, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   rat, monkey, feline, mouse, human, rabbit
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
mfr. no.   Chemicon®
NCBI accession no.   NM_003042.2
UniProt accession no.   P30531
Gene Information   human ... SLC6A1(6529)

Description

Specificity

Recognizes GABA Transporter-1 (GAT-1), C-terminus. No cross-reactivity to the C-termini of other transmitter transporters detected.

Immunogen

Rat GAT-1, C-terminus (aa 588-599)

Application

Anti-GABA Transporter-1 Antibody detects level of GABA Transporter-1 & has been published & validated for use in IH & WB.

Western blotting: 1:200-1:500 (67 kDa)

Immunohistochemistry

Optimal working dilutions must be determined by the end user.

Target description

67 kDa

Physical form

Affinity Purified antibody. Lyophilized from 0.1 M PBS with 1% BSA and 0.1% sodium azide. Reconstitute with 100 μL of sterile distilled water.

Storage and Stability

Maintain lyophilized material at 2–8°C for up to 12 months. Maintain reconstituted materialat -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Hazard statements 
Precautionary statements 
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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