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  • AB1585 - Anti-Dopamine β Hydroxylase Antibody

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AB1585 Sigma-Aldrich

Anti-Dopamine β Hydroxylase Antibody

serum, clone, Chemicon®

Synonym: DBH, Dopamine beta-monooxygenase

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, DM-DP, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable
  radioimmunoassay: suitable
  western blot: suitable
species reactivity   feline, human, rat, rabbit, guinea pig, bovine
shipped in   wet ice
antibody form   serum
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_000787.3
UniProt accession no.   P09172

Description

Specificity

Recognizes Dopamine beta hydroxylase. AB1585 reacts with a single band on Western blots of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with enzyme.

Immunogen

Native enzyme from bovine adrenal glands.

Application

Detect Dopamine β Hydroxylase using this Anti-Dopamine β Hydroxylase Antibody validated for use in IH, FUNC, RIA & WB.

Immunohistochemistry: 1:2,000 on rabbit and guinea pig using an ABC detection method.

Western blot

RIA

Inhibitory studies in vivo and in vitro.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Target description

69 kDa

Physical form

Rabbit Serum. Lyophilized, no preservatives. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any insoluble material.

Unpurified

Storage and Stability

Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles. Glycerol can be added 1:1 for additional stability.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Linkage

Replaces: AB145

Analysis Note

Control
Brain (Striatum/Substantia Nigra)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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