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  • AB19016 - Anti-MMP-9 Antibody, Catalytic domain

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AB19016 Sigma-Aldrich

Anti-MMP-9 Antibody, Catalytic domain

clone, Chemicon®, from rabbit

Synonym: Gelatinase B, 92 kDa Type IV Collagenase

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, MF-MN, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human, mouse, rat, mouse, rat, human
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NP_004985
UniProt accession no.   P14780
Gene Information   human ... MMP9(4318)

Description

General description

All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM.
MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III, and is produced by normal alveolar macrophages and granulocytes. The expression of MMP9 increases in Epstein-Barr virus infected lymphoma derived cell lines and may be of significance in typically invasive nasopharyngeal carcinomas.

Specificity

Reactivity with other species has not been confirmed.

Recognizes rat MMP-9 pro and active forms. Antibody also reacts with mouse MMP-9 and human MMP-9, other species not tested. Exhibits no cross-reactivity with other MMP family members. The antibody was generated using E. coli-expressed active rat 92 kDa type IV collagenase (catalytic domain) as an immunogen.

Immunogen

E. coli-expressed active rat 92 kDa type IV collagenase (catalytic domain)

Epitope: Catalytic domain

Application

Anti-MMP-9 Antibody, Catalytic domain is an antibody against MMP-9 for use in IH, IP & WB.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Western Blot Analysis:
1:1000 dilution of a previous lot was used.

Immunoprecipitation:
1:500 dilution from a previous lot was used. MMP-9 is constitutively produced by some tumor cell lines (i.e., HT1080, HL60, and U937), but not in most quiescent cells and tissues. The phorbol ester TPA stimulates production of MMP-9 in some cell types. MMP-9 levels in culture media (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, G.I. et al. J. Biol. Chem. 267, 4583-4591. 1992). This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media.
Western blot: 1:2,000. Typically 98-95 kDa pro and 92-88kDa active forms are detected as well as breakdown products under fully reduced conditions. Under non-reduced conditions additional bands may be detected because of SDS-stable TIMP and other inhibitor complexes. CC069 is recommended as a positive control.

Immunohistochemistry: 1:100 dilution from a previous lot was used in frozen acetone fixed tissues.

Optimal working dilutions must be determined by end user.

Target description

92 kDa

Physical form

Format: Purified

Protein A Purfied

Purified rabbit polyclonal in buffer containing PBS, containing 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality

Routinely evaluated by Western Blot on L6 lysates.

Western Blot Analysis: 1:1000 dilution of this lot detected MMP-9 on 10 µg of L6 lysates.

Linkage

Replaces: 04-1150

Analysis Note

Control
HL60 cell lysate, U937 cell lysate Lung.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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