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AB1936 Sigma-Aldrich

Anti-Integrin α2 Antibody

serum, clone, Chemicon®

Synonym: CD49b

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, IN-IP, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   ELISA: suitable
  immunofluorescence: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  radioimmunoassay: suitable
  western blot: suitable
species reactivity   mouse, chicken, hamster, rat, human
shipped in   wet ice
antibody form   serum
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_002203.3
UniProt accession no.   P17301
Gene Information   human ... ITGA2(3673)

Description

Specificity

Human alpha2 integrin subunit. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine labeled endothelial cells. The epitope recognized is on the cytoplasmic domain of the protein and is thus intracellular. Shows no cross-reactivity to alpha1, alpha3, alpha4, alpha5, alpha6, or alphaV subunits.

Immunogen

Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha2 integrin subunit {KYEKMTKNPDEODETTELSS}

Application

Anti-Integrin α2 Antibody detects level of Integrin α2 & has been published & validated for use in ELISA, IF, IH, IP, RIA & WB.

Immunofluorescence: 1:500 (suggested for use on acetone-fixed tissue only)

ELISA/RIA: 1:1000

Western blot: 1:1000 The antibody has been used to report alpha2 expression in HUVECs during tubule-like structure formation in collagen (Deroanne, 1996). Recognizes ~160kDa alpha 2 subunit

Immunoprecipitation: 5 μL precipitates alpha2/beta1 from 5 x 106 cells.

Immunohistochemistry: 1:500

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Target description

160kDa

Physical form

Neat rabbit antiSerum. Liquid with 0.05% sodium azide

Unpurified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
Skin (basement membrane), High grade ovarian tumors

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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