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  • AB2963 - Anti-MMP-3 Antibody, NT

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AB2963 Sigma-Aldrich

Anti-MMP-3 Antibody, NT

clone, from rabbit, purified by affinity chromatography

Synonym: matrix metallopeptidase 3 (stromelysin 1, progelatinase), matrix metalloproteinase 3 (stromelysin 1, progelatinase), Matrix metalloproteinase-3, transin-1, proteoglycanase, stromelysin-1, Transin-13

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, MF-MN, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   human, chimpanzee, rhesus macaque, human
species reactivity (predicted by homology)   rhesus monkey (100% sequence homology), chimpanzee (100% sequence homology)
shipped in   ambient
packaging   antibody small pack of 25 μg
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_002413
UniProt accession no.   P08254
Gene Information   human ... MMP3(4314)

Description

General description

MMP-3 (matrix metalloproteinase-3), also known as stromelysin-1, is a member of the matrix metalloproteinase (MMP) family of zinc endopeptidases. MMP’s are required for removal of extracellular matrix machinery during embryo development, morphogenesis and tissue remodeling. Endogenous tissue inhibitors of metalloproteinases (TIMPs) are responsible for regulating MMP-3. Any disruption of this system can cause diseases such as arthritis, metastasis, atherosclerosis and tumor growth.

Specificity

Other homologies: Mouse and rat (64% sequence homology).

This antibody recognizes MMP-3 at the N-terminus.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to human N-terminal MMP-3.

Application

Detect MMP-3 using this Anti-MMP-3 Antibody, N-terminus validated for use in WB, IH(P).

Immunohistochemistry Analysis: A 1:600 dilution from a previous lot detected MMP-3 in colorectal carcinoma and ductal carcinoma tissues.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Target description

~58 kDa observed.
An uncharacterized band may be seen at ~78 kDa

Physical form

Affinity Purfied

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in PMA-treated HL-60 concentrated media.

Western Blot Analysis: 0.1-1 µg/mL of this antibody detects MMP-3 in 10 µg of PMA-treated HL-60 concentrated media.

Linkage

Replaces: AB812

Analysis Note

Control
PMA-treated HL-60 concentrated media

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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