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AB3075 Sigma-Aldrich

Anti-Aquaporin 7 Antibody

Detect Aquaporin 7 using this Anti-Aquaporin 7 Antibody validated for use in ELISA & WB.

Synonym: AQP7

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Properties

Related Categories Antibodies, Primary Antibodies More...
trade name   Chemicon
brand family   Chemicon
format   Affinity Purified
application(s)   ELISA
  Western Blotting
clone   Polyclonal Antibody
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
host   Rabbit
species reactivity   Rat
NCBI accession no.   NM_001170.1
UniProt accession no.   O14520
gene symbol   AQP7(364)
  AQP9(366)
  AQP-7
  AQP7L
  AQPAP
  AQUAPORIN-7-LIKE
  AQUAPORIN-7
  MGC149555
  MGC149556
material size   50 µg
shipped in   wet ice
storage conditions   Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt.

Description

Background information

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26 kDa), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Aquaporin-7 (rAQP7, 269aa) is abundantly expressed in testis. It is involved in water, glycerol and urea transport. Aquaporin-7 is also found in adipose tissue, kidney, and heart. Aquaporin-7 has been localized to in the spermatids and at maturing sperms. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.

Physical form

Affinity Purified 1 mg/ml soln in PBS, pH 7.5 and 0.1% BSA and 0.05% sodium azide

Application

Note: We recommend the use of 0.5-1.0% milk in all primary/secondary antibody incubations to suppress non-specific reactivity.

Western blot: 1-10 μg/mL using Chemiluminescence technique

ELISA: 0.5-1.0 μg/mL when tested against 1 μg/mL of the immunogen peptide.

Immunohistochemistry: We recommend using the affinity purified antibody at 2-10 μg/mL in paraformaldehyde fixed sections of tissues.

Optimal working dilutions must be determined by end user.

Specificity

Aquaporin 7. The immunogen peptide is unique to Aquaporin-7 without significant homology to any other Aquaporins.

Immunogen

An 18 AA synthetic peptide within the N-terminal domain of rat Aquaporin-7 (Ishibashi et al. 1997) was selected for antibody production. This domain is predicted to be cytoplasmic.

Species Reactivity Notes

The peptide has 81% similarity with the mouse Aquaporin-7

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Making Headway
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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