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AB3091 Sigma-Aldrich

Anti-Aquaporin 9 Antibody

Detect Aquaporin 9 using this Anti-Aquaporin 9 Antibody validated for use in ELISA, IC, IH & WB.

Synonym: AQP9

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   50 µg (AB3091)
application(s)   ELISA: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
  western blot: suitable
biological source   chicken
clone   polyclonal
purified by   affinity chromatography
shipped in   dry ice
species reactivity   rat
gene symbol   AQP9(366)
trade name   Chemicon
brand family   Chemicon
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
gene symbol   AQP-9
  Aquaporin-9
  HsT17287
  SSC1

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Application

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blot: 1-10 μg/mL using ECL

ELISA: 1:100,000 using 50-100 ng of control peptide per well.

Immunohisto/cytochemistry: We recommend using the affinity purified antibody at 2-10 μg/mL in paraformaldehyde fixed sections of tissues. Adherent cells can be fixed with 50% methanol-50% acetone or 1% paraformaldehyde.

Optimal working dilutions must be determined by end user.

General description

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26 kDa), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane.

A new member of AQP family, Aquaporin-9 (AQP9), has been cloned from human leukocytes by homology cloning (Ishibashi et al. 1997). It is mostly homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295 AA) is primarily expressed in peripheral leukocytes, and lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it did not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.

Immunogen

An 18 AA synthetic peptide within the C-terminal domain of rat Aquaporin-9 (Ishibashi et al. 1997) was selected for antibody production. This domain is predicted to be cytoplasmic.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Specificity

Aquaporin 9. The immunogen peptide is unique to Aquaporin-9 without significant homology to any other Aquaporins.

Rat. The immunogen peptide has 94% conservation with mouse and 72% conservation with the human Aquaporin-9. Species reactivity has not been confirmed.

Physical form

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt.

Avoid repeated freeze/thaw cycles.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Making Headway
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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