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AB3383-I Sigma-Aldrich

Anti-UTF1 Antibody

clone, from rabbit, purified by affinity chromatography

Synonym: UTF1, Undifferentiated embryonic cell transcription factor 1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, UH-UZ
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   rat, rat, mouse, mouse
species reactivity (predicted by homology)   human (based on 100% sequence homology)
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_003568
UniProt accession no.   Q5T230

Description

General description

Undifferentiated embryonic cell transcription factor 1 is a nucleus protein encoded by the UTF1 gene in human and functions as a transcriptional coactivator of ATF2. UTF1 is reported to interact with the ATF-2 metal binding motif via the leucine zipper motif within its conserved domain 2 (CD2) and both CD1 & CD2 of UTF1 and also independently mediates association with TATA-binding protein-containing complex. (PMID 9749258)

Specificity

Other homologies: mouse, rat 95%.

Immunogen

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to human UTF1 near N-terminus.

Application

Anti-UTF1 Antibody is an antibody against UTF1 for use in Western Blotting and Immunohistochemistry.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected UTF1 in mouse kidney and rat liver tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Target description

~48 kDa observed. UTF1, a novel transcriptional coactivator expressed in pluripotent embryonic stem cells and extra-embryonic cells (Okuda, A., et al. (1998). EMBO. 17(7):2019–2032).

Physical form

Affinity

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in mouse embryonic stem cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected UTF1 in 10 µg of mouse embryonic stem cell lysate.

Linkage

Replaces: AB3383

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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