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AB3478 Sigma-Aldrich

Anti-Defensin β3 Antibody

Chemicon®, from rabbit

Synonym: BD-3, hBD-3

  •  eCl@ss 32160702

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Properties

Related Categories Alphabetical Index, Antibodies, DE-DL, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   ELISA: suitable
  western blot: suitable
species reactivity   human
shipped in   dry ice
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_018661.3
UniProt accession no.   P81534
Gene Information   human ... DEFB103B(414325)

Description

General description

Beta Defensin-3 is a novel 5 kDa nonhemolytic antimicrobial protein peptide which may be important in the innate epithelial defense of infections by various microorganisms seen in skin and lung (such as cystic fibrosis).

Specificity

Recognizes the 5-6 kDa human Defensin-3, beta protein.

Immunogen

A synthetic peptide consisting of amino acids 23-33 [GIINTLQKYYC] of the human beta-defensin-3 protein.

Application

Anti-Defensin Antibody, β3 is an antibody against Defensin for use in ELISA & WB.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Western blotting: Tricine-SDS- PAGE gels recommended: 1:500-1:1,000, migrates as a 5-6kDa band; Tricine-SDS-urea PAGE gels: Native Beta Defensin 3 migrates as a 9kDa band; the larger size is because of the reduction of the three cysteine bridges present in the peptide protein

Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified

Purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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