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  • AB3549P - Anti-Monocarboxylate Transporter 8 Antibody

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AB3549P Sigma-Aldrich

Anti-Monocarboxylate Transporter 8 Antibody

Chemicon®, from rabbit

Synonym: MCT8, X-linked PEST-containing Transporter

  •  eCl@ss 32160702

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Properties

Related Categories Alphabetical Index, Antibodies, MO-MR, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   ELISA: suitable
  western blot: suitable
species reactivity   mouse
shipped in   dry ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
mfr. no.   Chemicon®
NCBI accession no.   NM_006517.2
UniProt accession no.   P36021
Gene Information   human ... SLC16A2(6567)

Description

General description

MCT8 is an integral membrane protein that is associated with the plasma membrane. A member of the Major Facilitator Superfamily, MCT8 serves as a proton-linked monocarboxylate transporter that catalyzes the rapid transport of monocarboxylates and ketone bodies across the plasma membrane. Expression of MCT8 is greatest in liver and kidney.

Specificity

Recognizes mouse Monocarboxylate Transporter 8.

Immunogen

A 19 amino acid peptide sequence within the cytoplasmic, C-terminus of mouse MCT8. The immunogen sequence is 94% conserved in rat and 89% in human MCT8 and shows no significant sequence homology with other MCT family members.

Application

Anti-Monocarboxylate Transporter 8 Antibody is an antibody against Monocarboxylate Transporter 8 for use in ELISA & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blot: 1-5 μg/mL using ECL.
ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide (Catalog number AG202) per well.

Optimal working dilutions must be determined by the end user.

Physical form

Affinitypurified immunoglobulin presented as a liquid in PBS with 0.1% BSA.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB3324P

Analysis Note

Control
POSITIVE CONTROL: Liver or kidney extracts

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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