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AB3796 Sigma-Aldrich

Anti-5′-Lipoxygenase Antibody

Anti-5'-Lipoxygenase Antibody detects level of 5'-Lipoxygenase & has been published & validated for use in ELISA, IP & WB.

Synonym: Arachidonate 5-lipoxygenase, 5-LO

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   50 µL (AB3796)
gene symbol   ALOX5(240)
trade name   Chemicon
brand family   Chemicon
format   Purified
application(s)   ELISA
  Immunoprecipitation
  Western Blotting
clone   Polyclonal Antibody
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
host   Rabbit
species reactivity   Human
  Mouse
  Rabbit
  Rat
NCBI accession no.   NM_000698.2
UniProt accession no.   P09917
gene symbol   5-LO
  5-lipoxygenase
  5LPG
  EC 1.13.11.34
  LOG5
  MGC163204
shipped in   2

Description

Analysis Note

Control
POSITIVE CONTROL: placenta, lung, and leukocytes.

Application

Western blot: 1:1,000. Reacts with a 79 kDa band
Immunoprecipitation: 5 μL/mL lysate
ELISA: 1:1000 – 1:100,000
Optimal working dilutions must be determined by the end user.

General description

5′-Lipoxygenase is a critical enzyme in the formation of leukotrienes from arachidonic acid. Upon activation, the enzyme translocates to either a nuclear or plasma membrane compartment.

Immunogen

Purified human 5′-Lipoxygenase

Specificity

5′-Lipoxygenase

Physical form

Purified immunoglobulin in PBS with 50% Glycerol and 0.02% sodium azide

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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