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AB5054 Sigma-Aldrich

Anti-Calretinin Antibody

This Anti-Calretinin Antibody is validated for use in IH, IH(P), WB for the detection of Calretinin.

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Antibodies for Neurobiology, Antibodies to Neural Proteins, Neuroscience, Primary Antibodies More...
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable (paraffin)
  immunohistochemistry: suitable
  western blot: suitable
antibody form   serum
packaging   100 µL (AB5054)
NCBI accession no.   NM_001740.3
UniProt accession no.   P22676
mfr. no.   Chemicon
Gene Information   human ... CALB2(794)
species reactivity   human, rat, rat, human
shipped in   dry ice

Description

General description

Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc.

Specificity

Reactivity with other species has not been determined

Specific for calretinin. Recognizes both the calcium-bound and calcium-unbound conformations of calretinin by western blots.

Immunogen

Recombinant rat calretinin.

Application

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

Western blot:
1:1,000-1:2,000 of a previous lot was used.

Immunohistochemistry:
1:1,000-1:10,000
Antibody works in paraffin embedded tissues.

Optimal working dilutions must be determined by the end user.

Physical form

Rabbit polyclonal serum contains no preservative.

Unpurified

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
Rat brain cortex, Mesothelioma tissue, Human cerebellum.

Linkage

Replaces: AB149

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Immunohistochemistry(paraffin) Analysis:
Calretinin (cat. # AB5054) staining on Human Cerebellum. Tissue pretreated with Citrate, pH 6.0 plus. Antibody was diluted to 1:200 (5.0 µg/mL), using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as a nuclear and cytoplasmic punctate staining on neurons (brown).
Optimal Staining With Citrate Buffer Epitope Retrieval: Human Cerebellum.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Making Headway
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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