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  • AB5058 - Anti-Prion Protein Antibody, NT, a.a. 78-97

AB5058 Sigma-Aldrich

Anti-Prion Protein Antibody, NT, a.a. 78-97

Anti-Prion Protein Antibody, N-terminus, a.a. 78-97 detects level of Prion Protein & has been published & validated for use in ELISA, WB, IH(P).

Synonym: PrP, CD230

  •  eCl@ss 32160702



Related Categories Alphabetical Index, Antibodies, PR-PR, Primary Antibodies
clone   polyclonal
biological source   goat
application(s)   ELISA: suitable
  immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   human
shipped in   dry ice
antibody form   serum
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_000311.3
UniProt accession no.   P04156
Gene Information   human ... PRNP(5621)



Specific for human prion protein (PrP). This antibody immunolabels amyloid plaques in formalin-fixed paraffin sections from Creutzfeld-Jakob Disease (CJD) brain.The prion protein is a large membrane protein that occurs normally in neurons of the human brain and is thought to be involved in synaptic transmission. In prion diseases, such as CJD, Gerstmann-Straussler-Scheinker syndrome (GSS), Fatal Familial Insomnia (FFI), Alpers Syndrome and Kuru, the normal cellular form of this protein (PrPc) is transformed into an altered protein when it comes into contact with an infectious prion protein (PrPsc) from another host. This altered PrPsc accumulates in cytoplasmic vesicles of diseased individuals forming lesions, vacuoles and amyloid deposits.


Epitope: N-terminus, a.a. 78-97

Synthetic peptide that corresponds to amino acids 79-97 of the N-terminus of the human PrP27-30.


Immunohistochemistry: >1:200 on paraffin embedded, formalin fixed human brain.

Western blots: > 1:2,000

ELISA: > 1:35,000

Optimal working dilutions must be determined by the end user.

Research Category

Research Sub Category
Neurodegenerative Diseases

Physical form

Goat serum. Liquid containing 0.01% thimerosal

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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