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  • AB5150 - Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT

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AB5150 Sigma-Aldrich

Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT

clone, Chemicon®, from rabbit

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, CA1-CAL, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse, rat
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
mfr. no.   Chemicon®
NCBI accession no.   NM_000719.5
UniProt accession no.   Q13936
Gene Information   human ... CACNA1C(775)

Description

Specificity

Recognizes all types of alpha1 subunits of voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far. The epitope recongnized by the AB5150 is masked by alpha2 and delta chains, so this antibody can bind only after using Triton X 100 or other detergents that cause subunit dissociation (Ahlijanian et al. 1991).

Immunogen

Purified peptide from {C-DNFDYLTRDWSILGPHHLD [CP15], cooresponding to residues 1506-1524 of rat alpha-1C {Accession P2202} subunit of skeletal muscle voltage-gated calcium channel (Striessnig et al. 1990), conjugated to KLH (Harlow & Lane 1988).

Application

Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, C-terminus detects level of Calcium Channel & has been published & validated for use in IC, IP & WB.

In preparing full-length protein samples for Western blot or immunoprecipitation, the procedures should be performed at 4°C, with the following protease inhibitor mixture: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each) (Westenbroek et al. 1992).

Western blot: 1:200 using ECL on rat brain membranes.

Immunohistochemistry using rat heart sections. Note: the epitope can be masked by other subunits in some Ca2+ channels.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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