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AB5220 Sigma-Aldrich

Anti-Growth Associated Protein-43 (GAP-43) Antibody

Anti-Growth Associated Protein-43 (GAP-43) Antibody is an antibody against Growth Associated Protein-43 (GAP-43) for use in ELISA, IC, IH, IP & WB.

Synonym: Neuromodulin

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, GQ-GZ, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   ELISA: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse (high degree), bovine, mouse, chicken, human, rat
shipped in   dry ice
antibody product type   primary antibodies
Quality Level   100
mfr. no.   Chemicon®
NCBI accession no.   NM_002045.2
UniProt accession no.   P17677
Gene Information   human ... GAP43(2596)

Description

General description

GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.

Specificity

Reacts to a lesser degree with rat, human, bovine and chicken. Other species have not been tested.

Recognizes Growth Associated Protein-43 (GAP-43).

Immunogen

Recombinant rat GAP-43 (complete sequence).

Application

Immunohistochemistry:
1:1,000 dilution of a previous lot was used on 4% paraformaldehyde / PBS fixed or 3% Paraformaldehyde / 0.1% Gluteraldehyde / PBS fixed tissue sections.

Immunocytochemistry:
1:1,000 dilution of a previous lot was used on Hippocampal neurons in culture and PC12 cells stimulated with NGF.

Immunoprecipitation:
1:100 dilution of a previous lot was used.

ELISA:
1:2,000 dilution of a previous lot was used.

Target description

23.6 kDa

Physical form

Format: Purified

Purified rabbit polyclonal in buffer containing PBS with 0.01% sodium azide as a preservative.

Storage and Stability

Stable for up to 6 months at -20ºC in undiluted aliquots from date of receipt.
Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.

Quality

Routinely evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis: 1:500 dilution of this lot detected Growth Associated Protein 43 on 10 μg of Mouse brain lysates.

Analysis Note

Control
Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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