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  • AB5320 - Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody

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AB5320 Sigma-Aldrich

Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody

Synonym: Chondroitin sulfate proteoglycan NG2, Melanoma chondroitin sulfate proteoglycan 3, Melanoma-associated chondroitin sulfate proteoglycan, chondroitin sulfate proteoglycan 4, chondroitin sulfate proteoglycan 4 (melanoma-associated)

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, NE-NI, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   ELISA: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse, rat, human, monkey
shipped in   dry ice
antibody product type   primary antibodies
Quality Level   100
purified by   affinity chromatography
mfr. no.   Chemicon®
NCBI accession no.   NM_001897.4
UniProt accession no.   Q6UVK1

Description

General description

Chondroitin sulfate proteoglycan 4 (UniProt: Q00657; also known as Chondroitin sulfate proteoglycan NG2, HSN tumor-specific antigen) is encoded by the Cspg4 (also known as Ng2) gene (Gene ID: 81651) in rat. Chondroitin sulfate proteoglycan NG2 is a single-pass type I membrane protein that plays a role in cell proliferation and migration and stimulates endothelial cells motility during microvascular morphogenesis. It is present in neural cells and in extraneural tissues, especially in the developing mesenchyme. Its level of expression is highest on immature, proliferating cells and decreases when these cells begin to differentiate. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells. It is a major chondroitin sulfate proteoglycan that is produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors. It is also reported to inhibit neurite outgrowth and growth cone collapse during axon regeneration. NG2 chondroitin sulfate proteoglycan is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off in the mature form.(Ref.: Jones, LL et al. (2002). J. Neurosci. 22(7):2792-2803).

Specificity

NG2 Chondroitin Sulfate Proteoglycan. AB5320 identifies both the intact proteoglycan and the core protein by Western blot and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well.

Immunogen

Immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat.

Application

Immunocytochemistry:
A 1:150-1:600 dilution of a previous lot was used in IC.

Immunohistochemistry:
1:200 dilution of a previous lot was used on frozen sections of embryonic mouse brain using an Alexa Fluor conjugated secondary antibody. Care should be taken to avoid overfixing tissue sections. A short (5-10 minute) fix in 2-4% PFA is recommended.

Immunoprecipitation:
2 μg/mL concentration of a previous lot was used in IP.

ELISA:
A 1:1,500-1:3,000 dilution of a previous lot was used in ELISA.

Optimal working dilutions and protocols must be determined by end user

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Target description

270-300 kDa

Physical form

Antibody purified by protein A, then placed over a fibronectin affinity column.

Purified rabbit polyclonal in PBS buffer containing 0.02% azide.

Storage and Stability

Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Routinely evaluated by Western Blot on rat brain lysates

Western Blot: 1:1000 dilution of this lot detected NG2 chondroitin sulfate on 10 μg of rat brain lysates.

Analysis Note

Control
Human melanoma, glioma and proliferating brain endothelial cells, rat B49 glial cell extracts or embryonic mouse brain tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Chondroitinase for Neural Regeneration Research

The spinal cord is the information highway system for the brain. And axons are the road that allows information data to be transferred. So, when a traumatic spinal cord injury (SCI) occurs and axons ...
Keywords: Catalysis, Cell culture, Degradations, High performance liquid chromatography, Immunofluorescence, PAGE

Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Introduction Methods    Human Neural Stem Cell Culture    Oligodendrocyte Differentiation and Maturation    Oligodendrocyte Progenitor Cell Characterization    In Vitro Myelination Assay Results Conc...
Christine Chen, Michael Moeller, Anna Abai, Nick Asbrock and Vi Chu1
1
MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Adhesion, Atomic absorption spectroscopy, Cell culture, Cell proliferation, Central Nervous System, Clinical, Culture media, Diseases, Gene expression, Growth factors, Hormones, Neurodegenerative Diseases, Pharmaceutical, Phase transitions, RNA immunoprecipitation, Transfection

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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