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AB5354 Sigma-Aldrich

Anti-Internexin α Antibody

Anti-Internexin Antibody, α is an antibody against Internexin for use in ELISA, WB, IC, IH.

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, IN-IP, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   ELISA: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity (predicted by homology)   mammals
shipped in   dry ice
antibody form   serum
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_032727.2
UniProt accession no.   Q16352
Gene Information   human ... INA(9118)

Description

Specificity

alpha-internexin, C-terminal globular "tail" region. By western blot, AB5354 detects a major band at 66 kDa and a minor band at approximately 150 kDa corresponding to alpha internexin monomer and dimer, respectively. A minor band at approximately 50 kDa is occasionally detected and is believed to be a degradation product of alpha internexin. This antibody has been referred to in the literature as the R36 antibody (Evans, 2002).

Immunogen

Full length recombinant rat alpha-internexin fused to E. coli Trp E.

Application

Immunohistochemistry on formalin or paraformaldehyde fixed tissues.

Immunocytochemistry.

Western blot: using human or rat brain homogenates.

ELISA

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Physical form

Serum liquid, containing no preservatives.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Linkage

Replaces: 04-1032

Analysis Note

Control
POSITIVE CONTROL CELLS: PC12, Ntera2.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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