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  • AB5374 - Anti-Potassium Channel Kir2.1 Antibody

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AB5374 Sigma-Aldrich

Anti-Potassium Channel Kir2.1 Antibody

Chemicon®, from rabbit

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, POD-POZ, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   rat
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
mfr. no.   Chemicon®
NCBI accession no.   NM_000891.2
UniProt accession no.   P63252
Gene Information   human ... KCNJ2(3759)

Description

Specificity

Recognizes Kir2.1 protein (IRK1, Kcnj2). The epitope is specific for Kir2.1 and is not present in any other known protein.

SPECIES REACTIVITIES: The immunogen sequence is identical in rabbit, bovine, porcine and guinea pig. It is highly conserved in rat and mouse (17/19 residues), chick (15/19 residues), and pigeon (14/18). Other species have not been tested.

Immunogen

Purified peptide from human Kir2.1 (amino acids 392-410) (Accession number P48049).

Application

Detect Potassium Channel Kir2.1 using this Anti-Potassium Channel Kir2.1 Antibody validated for use in IH & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blot: 1:200 using ECL on rat brain and rat heart membranes. Note: Addition of 0.1% Tween to the standard milk block is recommended for this lot.

Immunohistochemistry on rat heart sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Analysis Note

Control
CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Hazard statements 
Precautionary statements 
WGK Germany 
WGK 3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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