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AB5535 Sigma-Aldrich

Anti-Sox9 Antibody

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SO-SO
clone   polyclonal
biological source   rabbit
application(s)   ChIP: suitable (ChIP-seq)
  ChIP: suitable
  immunocytochemistry: suitable
  immunofluorescence: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   chicken, rat, chicken, mouse, mouse, rat, human, human
species reactivity (predicted by homology)   sheep (based on 100% sequence homology), equine (based on 100% sequence homology), feline (based on 100% sequence homology), bovine (based on 100% sequence homology)
shipped in   ambient
antibody product type   primary antibodies
purified by   affinity chromatography
mfr. no.   Chemicon®
NCBI accession no.   NP_000337
UniProt accession no.   P48436
Gene Information   human ... SOX9(6662), SRA1(10011)

Description

General description

Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at a minimum of 40 different loci that rapidly diverged in various animal lineages. At present 30 Sox genes have been identified, and members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.

Specificity

Recognizes Sox9.

Immunogen

Epitope: C-terminal

KLH-conjugated linear peptide corresponding to the C-terminal sequence of human Sox9.

Application

Anti-Sox9 Antibody is a well characterized affinity purified Rabbit Polyclonal Antibody that reliably detects Transcription Factor Sox-9. This highly published antibody has been validated in IHC & WB.

Immunohistochemistry Analysis: An 1:1,000 dilution from a representative lot detected Sox9 in murine embryonic bone and adult cartilage tissue sections.
Western Blotting Analysis: An 1:500 dilution from a representative lot detected Sox9 in human PC3 prostate cancer cells and HepG2 hepatocytes.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Sox9 occupancy at target chromatin sites by ChIP using chromatin preparations from P1 post-natal mouse rib chondrocytes (Ohba, S., et al. (2015). Cell Rep. 12(2):229-243).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Sox9 occupancy at the Bmi promoter in Z/sox9tg but not in wild-type control mouse embryonic fibroblasts/MEFs (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315).
ChIP-sequencing (ChIP-seq) Analysis: A representative lot detected Sox9-targeted chromatin sites by a genome-wide ChIP-seq analysis using chromatin preparations from P1 post-natal mouse rib chondrocytes (Ohba, S., et al. (2015). Cell Rep. 12(2):229-243).
Immunofluorescence Analysis: A representative lot detected the accumulation of Sox9-positive oval cells by fluorescent immunohistochemistry staining of paraffin-embedded liver sections from transgenic mice treated with diethylnitrosamine to induce conditional liver HNF4a knockout (Saha, S.K., et al. (2014). Nature. 513(7516):110-114).
Immunofluorescence Analysis: Representative lots detected Sox9 immunoreactivity in paraffin-embedded mouse embryo sections by fluorescent immunohistochemistry (Carrasco, M., et al. (2012). J. Clin. Invest. 122(10):3504-3515; Sylva, M., et al. (2011). PLoS One. 6(8):e22616).
Immunofluorescence Analysis: Representative lots immunostained Müller glial cells in frozen mouse and chicken retina sections by fluorescent immunohistochemistry staining of (Muranishi, Y., and Furukawa, T. (2012). J. Biomed. Biotechnol. 2012:973140; Fischer, A.J., et al. (2011). Neuroscience. 178:250-260).
Immunocytochemistry Analysis: A representative lot detected the stem cell marker Sox9 by fluorescent immunocytochemistry staining of paraformaldehyde-fixed E-Cad/Lgr6+ human lung stem cells (HLSCs) clonally derived and passaged in culture (Oeztuerk-Winder, F., et al. (2012). EMBO J. 31(16):3431-3441).
Immunohistochemistry Analysis: A representative lot immunostained the supporting cells (Sertoli) of the seminiferous tubules by immunohistochemistry staining of paraffin-embedded mouse testis sections (O′Shaughnessy, P.J., et al. (2012). PLoS One. 7(4):e35136).
Immunohistochemistry Analysis: A representative lot detected Sox9 immunoreactivity in various formalin-fixed, paraffin-embedded human tumor tissue sections (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315).
Western Blotting Analysis: A representative lot detected the stem cell marker Sox9 in E-Cad/Lgr6+ human lung stem cells (HLSCs) clonally derived and passaged in culture (Oeztuerk-Winder, F., et al. (2012). EMBO J. 31(16):3431-3441).
Western Blotting Analysis: A representative lot detected upregulated Sox9 expression level in human colorectal cancer cell lines, HCT116, DLD1, and SW620 (Matheu, A., et al. (2012). Cancer Res. 72(5):1301-1315).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Target description

56-65 kDa

Physical form

ImmunoAffinity Purified

Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide

Storage and Stability

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: An 1:2000 dilution of this antibody detected Sox9 in HepG2 cell lysate.

Linkage

Replaces: AB5809

Analysis Note

Control
Embryonic tissue, Adult chondrocytes.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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