• USA Home
  • AB5699 - Anti-Cysteine-rich Motor Neuron 1 Antibody

EMAIL THIS PAGE TO A FRIEND
AB5699 Sigma-Aldrich

Anti-Cysteine-rich Motor Neuron 1 Antibody

serum, clone, Chemicon®

Synonym: CRIM1

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, CT-CZ, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human, mouse
shipped in   dry ice
antibody form   serum
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_016441.1
UniProt accession no.   Q9NZV1
Gene Information   human ... CRIM1(51232)

Description

Specificity

Crim1

SUBCELLULAR LOCALIZATION: Intracellular and Membrane associated.

Immunogen

Recombinant protein from the cytoplasmic tail of mouse Crim1.

Application

Anti-Cysteine-rich Motor Neuron 1 Antibody detects level of Cysteine-rich Motor Neuron 1 & has been published & validated for use in IP & WB.

Research Category
Neuroscience

Research Sub Category
Neurochemistry & Neurotrophins

Western blot: 1:1,000 using ECL. The antibody reacts with proteins of ~120 kDa. Preferred gel percentage is 7.5%. Suggested blocking buffer is PBS containing 5% non-fat milk. Suggested dilution buffer is PBS containing 1% non-fat milk.

Immunoprecipitation: 5 μL to 1 mL of cell extract. Preferred tissue/cell lysis buffer is RIPA. Suggested incubation time is 2-4 hours. Suggested capture agent is Protein A.

Optimal working dilutions must be determined by the end user.

Physical form

Rabbit serum. Liquid.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?