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  • AB5883 - Anti-Synapsin I Antibody, phosphoSer 603

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AB5883 Sigma-Aldrich

Anti-Synapsin I Antibody, phosphoSer 603

clone, Chemicon®, from rabbit

  •  eCl@ss 32160702

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SU-SY
clone   polyclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   rat
shipped in   dry ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
mfr. no.   Chemicon®
NCBI accession no.   NM_006950.3
  NM_133499.2
UniProt accession no.   P17600
Gene Information   human ... SYN1(6853)

Description

Specificity

Synapsin I, phosphoSer603. This is the site phosphorylated by Calcium calmodulin kinase II. The antibody recognizes the ~78 kD Synapsin I, phosphoSer603 protein. Immunolabeling is blocked by the immunogen peptide but no by the corresponding non-phospho peptide.

Immunogen

Epitope: phosphoSer 603

Synthetic peptide corresponding to amino acids surrounding the phosphoSer603 of rat Synapsin I. The immunogen sequence is identical in human, mouse and Xenopus.

Application

Anti-Synapsin I Antibody, phosphoSer 603 detects level of Synapsin I & has been published & validated for use in WB.

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

Western blot: 1:1,000. Rat brain lysate shows a ~78-80kDa band.

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin presented as a liquid in 10 mM HEPES, pH 7.5, with 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Storage and Stability

Maintain at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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