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AB5934 Sigma-Aldrich

Anti-Sox1 Antibody

Detect Sox1 using this Anti-Sox1 Antibody. Validated for use in WB & IC.

Synonym: Transcription factor SOX-1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SO-SO
clone   polyclonal
biological source   chicken
application(s)   western blot: suitable
species reactivity   rat, rat, human, mouse, mouse, human
species reactivity (predicted by homology)   rhesus macaque (based on 100% sequence homology)
shipped in   wet ice
antibody product type   primary antibodies
purified by   affinity chromatography
mfr. no.   Chemicon®
NCBI accession no.   NP_005977
UniProt accession no.   O00570
Gene Information   human ... SOX1(6656)

Description

General description

Sox-1 protein (SOX1), also known as SRY (Sex determining region Y)-box 1, is expressed in the developing brain. SOX1 is related to the HMG box of family transcription factors, which are a highly motile group, with a highly conserved DNA binding domain. Sox1 utilizes multiple pathways to play a direct role on cell fate. SOX1 is also directly involved with sex determination of the Y chromosome. Binding of the SOX1, C-terminus, to the Hes 1 promoter results in Hes1 suppression and attenuation of Notch signaling. When the C-terminus of SOX1 binds to β-catenin, TCF/LEF is suppressed, resulting in the attenuation of Wnt signaling. SOX1, therefore, defines the dividing neural precursors of the embryonic central nervous system.

Specificity

This antibody recognizes SOX1 near the N-terminus.

Immunogen

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to human SOX1 near the N-terminus.

Application

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Neuronal & Glial Markers

Target description

~39 kDa observed. An uncharacterized band may appear at ~110 kDa in some lysates.

Physical form

Affinity Purfied

Purified chicken IgY in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in mouse neuronal stem cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected SOX1 on 10 µg of mouse neuronal stem cell lysate.

Analysis Note

Control
Mouse neuronal stem cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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