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  • AB6001 - Anti-MMP-9 Antibody, NT

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AB6001 Sigma-Aldrich

Anti-MMP-9 Antibody, NT

clone, from rabbit, purified by affinity chromatography

Synonym: 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, macrophage gelatinase, matrix metallopeptidase 9, matrix metalloproteinase 9, type V collagenase

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, MF-MN, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   canine, horse, human, human, rat, primate, monkey, pig
species reactivity (predicted by homology)   rat (based on 100% sequence homology), primate (based on 100% sequence homology), monkey (based on 100% sequence homology), dog (based on 100% sequence homology), horse (based on 100% sequence homology), pig (based on 100% sequence homology)
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_004985.2
UniProt accession no.   P14780
Gene Information   human ... MMP9(4318)

Description

General description

All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn2+ binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases).

MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III and is produced by normal alveolar macrophages and granulocytes.

Specificity

The antibody recognizes human MMP-9. It does not cross react with MMP-1 or MMP-2.

Immunogen

Epitope: N-terminus

KLH-conjugated synthetic peptide corresponding to the N-terminus of MMP-9.

Application

Anti-MMP-9 Antibody, N-terminus is an antibody against MMP-9 for use in WB.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Target description

92kDa (pro-form) and 88kDa (active form)

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated on a representative lot by Western blot using an HL-60 cell lysate supplemented with a PMA conditioned media.

Western Blot Analysis: 0.5 µg/ml of this antibody detected MMP-9 on 10 µg of HL-60 plus PMA conditioned media.

Linkage

Replaces: AB805

Analysis Note

Control
HL-60 plus PMA conditioned media

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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