• USA Home
  • AB749P - Anti-Collagen Type I Antibody

EMAIL THIS PAGE TO A FRIEND
AB749P Sigma-Aldrich

Anti-Collagen Type I Antibody

clone, Chemicon®, from rabbit

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, CO-CP, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   ELISA: suitable
  immunohistochemistry: suitable (paraffin)
  immunohistochemistry: suitable
  radioimmunoassay: suitable
species reactivity   bovine
shipped in   dry ice
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
suitability   not suitable for Western blot
NCBI accession no.   NM_000088.3
UniProt accession no.   P02452
Gene Information   human ... COL1A1(1277)

Description

Specificity

Reacts with bovine collagen type I collagen.

Antibody shows less than 0.1% cross reactivity in RIA at 1:500 dilution with bovine collagen types II, IX & XI, or fibronectin; other collagens and ECM proteins untested.

Reactivity with human collagen type I < 2.0% with RIA, 1:500; reactivity with Chicken collagen type I, less than 0.1% by RIA at 1:500 dilution.

Immunogen

purified bovine type I collagen extracted from purified bovine skin.

Application

Anti-Collagen Type I Antibody detects level of Collagen Type I & has been published & validated for use in ELISA, RIA, IH, IH(P).

Immunohistochemistry: 1:40 dilution for immunofluorescent staining of frozen bovine liver and skin tissues.

Immunohistochemistry on 2% formalin or PFA fixed, paraffin embedded tissues requires HEIR antigen retrieval and enhanced enzymatic detection; methyl-carnoy, acetone, or acid-aldehyde fixation can enhance reactivity for paraffin sections.

Radioimmunoassay

ELISA: 1:200 direct

Not recommended for Western blots.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Physical form

Format: Purified

Protein G purified IgG fraction in liquid at 1 mg/mL in 0.01M phosphate, 0.09M NaCl, pH 7.2.No preservatives.

Storage and Stability

Maintain frozen at -20°C for up to 12 months. Avoid repeated freeze/thaw cycles

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Linkage

Replaces: AB749

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?