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AB755P Sigma-Aldrich

Anti-Rat Collagen Type I Antibody

clone, Chemicon®, from rabbit

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, RAE-RAX
clone   polyclonal
biological source   rabbit
application(s)   ELISA: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  radioimmunoassay: suitable
species reactivity   rat
shipped in   wet ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
mfr. no.   Chemicon®
suitability   not suitable for Western blot
NCBI accession no.   NM_000088.3
UniProt accession no.   P02452
Gene Information   human ... COL1A1(1277)

Description

General description

Collagen Type I extracted and purified from rat skin. Antibody shows less than 0.1% reactivity with human, mouse, chicken Collagen Type I, rat Collagen Types II, III and V and rat Elastin.

Specificity

Recognizes Rat Collagen Type I

The antibody shows less than 0.1% reactivity with human, mouse, chicken Collagen Type I, rat Collagen Types II, III and V and rat Elastin.

Immunogen

Collagen Type I extracted and purified from rat skin.

Application

Anti-Rat Collagen Type I Antibody is an antibody against Collagen Type I for use in ELISA, IC, IH(P), RIA.

ELISA:
A 1:200 dilution of a previous lot was used in ELISA.

RIA:
A previous lot of this antibody was used in RIA.

Immunocytochemistry:
A 1:40-1:80 dilution of a previous lot was used for immunofluorescent staining of frozen rat skin and liver tissues.
Can also be used for acetone-fixed cells.

Immunohistochemistry:
1:40-1:80 dilution for immunofluorescent staining of frozen rat skin and liver tissues. The antibody is also reactive on paraffin embedded rat tissues (skin, liver) at a tdilution of 1:500 using an ABC detection system.

Not recommended for Western blots.
Optimal working dilutions must be determined by the end user.

Physical form

Purified rabbit polyclonal. Contains no preservative.

Quality

Immunohistochemistry(paraffin):
Collagen Type I (cat. # AB755P) staining pattern/morphology in rat skin. Tissue is pre-treated with Citrate pH 6.0, antigen retrieval. This lot of antibody is diluted to 1:1000, using IHC-Select reagents used with HRP-DAB. Immunoreactivity is seen fiber-staining on paraffin section. Note that the staining pattern is as expected.

IHC-Paraffin Staining With Epitope Retrieval: Rat Skin

Analysis Note

Control
Rat hepatic stellate cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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