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  • AB758B - Anti-Collagen Type I Antibody, biotin conjugated

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AB758B Sigma-Aldrich

Anti-Collagen Type I Antibody, biotin conjugated

clone, Chemicon®, from goat

  •  eCl@ss 32160702

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Properties

Related Categories Alphabetical Index, Antibodies, CO-CP, Primary Antibodies
conjugate   biotin conjugate
clone   polyclonal
biological source   goat
application(s)   ELISA: suitable
  dot blot: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   human, bovine
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
suitability   not suitable for immunohistochemistry (Paraffin)
NCBI accession no.   NM_000088.3
UniProt accession no.   P02452
Gene Information   human ... COL1A1(1277)

Description

Specificity

Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758B has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

Immunogen

Human placental collagen type I

Application

Anti-Collagen Type I Antibody, biotin conjugated is an antibody against Collagen Type Id for use in DB, ELISA, WB, IH.

Dot and slot blotting: 1:500-1:1,000

ELISA: 1:1,000-4,000

Indirect immunohistochemistry (frozen sections only): 1:20-1:40

The non-biotinylated version (Cat. # AB758) reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts , and has been cited for use in WB applications (PMID: 20089870)

Not recommended for paraffin tissue sections.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Physical form

Affinity purified immunoglobulin, biotin labeled. Prior to purification the antisera was adsorbed against collagen type II, III, IV, V and VI immobilized on Sepharose® 4B.. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

Sepharose is a registered trademark of Cytiva

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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