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AB765P Sigma-Aldrich

Anti-Mouse Collagen Type I Antibody

clone, Chemicon®, from rabbit

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, MO-MR, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   ELISA: suitable
  immunohistochemistry: suitable
  radioimmunoassay: suitable
  western blot: suitable
species reactivity   mouse
shipped in   dry ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_000088.3
UniProt accession no.   P02452
Gene Information   human ... COL1A1(1277)

Description

General description

Collagen Type I extracted and purified from mouse skin. Antibody shows less than 0.1% reactivity with mouse Collagen Types II, IV and with human, rat and chicken Collagens Type I in addition to a 1.0% reactivity with mouse Collagen Type III.

Collagen is the main protein of connective tissue in animals and the most abundant protein in mammals, making up about 25% of the total protein content. It is one of the long, fibrous structural proteins whose functions are quite different from those of globular proteins such as enzymes; tough bundles of collagen called collagen fibers are a major component of the extracellular matrix that supports most tissues and gives cells structure from the outside, but collagen is also found inside certain cells. Collagen has great tensile strength, and is the main component of fascia, cartilage, ligaments, tendons, bone and teeth. Along with soft keratin, it is responsible for skin strength and elasticity, and its degradation leads to wrinkles that accompany aging. It strengthens blood vessels and plays a role in tissue development. It is present in the cornea and lens of the eye in crystalline form. Collagen occurs in many places throughout the body. There are 12 types of collagen described in literature. Collagen Type 1: This is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is found in tendons, the endomysium of myofibrils and the organic part of bone. Collagen Type 4: It is present in basal lamina and the eye lens. Also serves as part of the filtration system in capillaries and the glomeruli of nephron in the kidney.

Specificity

Antibody shows less than 0.1% reactivity with mouse Collagen Types II, IV and with human, rat and chicken Collagens Type I in addition to a 1.0% reactivity with mouse Collagen Type III.

Recognizes mouse Collagen Type I.

Immunogen

Collagen Type I extracted and purified from mouse skin.

Application

Anti-Mouse Collagen Type I Antibody detects level of Mouse Collagen Type I and has been published in over 20 citations and validated for use in ELISA, IH, RIA and WB.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Western Blot Analysis:
1:500 dilution of a previous lot detected Collagen Type I on 10 μg of Mouse Liver lysate.

Immunohistochemistry:
1:40 dilution of a previous lot for immunofluorescent staining of frozen mouse skin and liver tissues.

RIA:
A 1:200 dilution of a previous lot was used in RIA.

ELISA:
A previous lot of this antibody was used in ELISA

Target description

MW of Collagen Type I precursor: 140-210 kDa MW of mature Collagen Type I: 70-90 kDa

Physical form

Format: Purified

Protein A Purfied

Purified rabbit polyclonal serum in buffer containing 0.01 M phosphate, 0.09 M NaCl, pH 7.2. No preservatives.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Linkage

Replaces: AB765

Analysis Note

Control
Mouse skin and mouse liver tissues

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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