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AB769 Sigma-Aldrich

Anti-Collagen Type IV Antibody

clone, Chemicon®, from goat

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, CO-CP, Primary Antibodies
clone   polyclonal
biological source   goat
application(s)   ELISA: suitable
  dot blot: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
species reactivity   human, mouse, bovine
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
mfr. no.   Chemicon®
suitability   not suitable for Western blot
  not suitable for immunohistochemistry (Paraffin)
NCBI accession no.   NM_001845.4
UniProt accession no.   P02462
Gene Information   human ... COL4A1(1282)

Description

Specificity

Recognizes Human and bovine type IV collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types I, II, III, V, and VI. May show reactivity to type IV collagen from other species. AB769 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

Immunogen

Human and bovine placental collagen type IV

Application

Detect Collagen Type IV using this Anti-Collagen Type IV Antibody validated for use in DB, ELISA, IC, IH.

Dot and slot blotting: 1:100-1:500

ELISA: 1:1000-1:4000

Indirect immunohistochemistry (frozen sections only): 1:10-1:40

Immunocytochemistry 1:10-1:20

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Physical form

Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen type I, II, III, V and VI immobilized on Sepharose® 4B. Liquid in borate buffered saline, pH 8.2, no sodium azide.

ImmunoAffinity Purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

Sepharose is a registered trademark of Cytiva

Analysis Note

Control
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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