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AB9272 Sigma-Aldrich

Anti-Synaptophysin Antibody

clone, Chemicon®, from rabbit

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SU-SY
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   rat, human
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
mfr. no.   Chemicon®
NCBI accession no.   NM_003179.2
UniProt accession no.   P08247
Gene Information   human ... SYP(6855)

Description

Specificity

Synaptophysin

Immunogen

Synthetic peptide from human synaptophysin.

Application

Anti-Synaptophysin Antibody detects level of Synaptophysin & has been published & validated for use in WB, IH(P).

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

Western blot

Immunohistochemistry on formalin fixed, paraffin embedded tissue: 1:300 for 10 minutes at room temperature. Staining of formalin-fixed tissues requires boiling of tissues in 10 mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Optimal working dilutions must be determined by the end user.

STAINING PATTERN: Cytoplasmic

Physical form

Affinity purified immunoglobulin. Liquid in 10 mM PBS, pH 7.4 containing BSA and 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C for up to 6 months after date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Linkage

Replaces: 04-1019

Analysis Note

Control
POSITIVE CONTROL: Pancreas or Pheochromocytoma

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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