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AB9610 Sigma-Aldrich

Anti-Olig-2 Antibody

clone, Chemicon®, from rabbit

Synonym: Oligodendrocyte transcription factor 2, Oligo2, Class B basic helix-loop-helix protein 1, bHLHb1, Class E basic helix-loop-helix protein 19, bHLHe19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, O, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse, rat, rat, human, human, mouse
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_005806.2
UniProt accession no.   Q13516
Gene Information   human ... OLIG2(10215)

Description

General description

Oligodendrocyte transcription factor 2 (UniProt: Q13516, also known as Oligo2, Class B basic helix-loop-helix protein 1, bHLHb1, Class E basic helix-loop-helix protein 19, bHLHe19, Protein kinase C-binding protein 2, Protein kinase C-binding protein RACK17) is encoded by the OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) gene (Gene ID: 10215) in human. Olig2 is expressed in the brain in oligodendrocytes. Its strong expression is also observed in oligodendrogliomas. Olig2 is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. It functions together with ZNF488 to promote oligodendrocyte differentiation. Olig2 is observed in both nucleus and the cytoplasm. Its nuclear localization signal is contained in the bHLH domain (aa 108-162). It can be masked in the native form and translocation to the nucleus may be mediated by interaction either with class E bHLH partner protein or with NKX2-2. Olig2 cooperates with Olig1 to establish the motor neuron progenitor domain of the embryonic neural tube. Removal of Cdx transcription factors is shown to result in the continued induction of Olig2 and ectopic production of cranial motor neuron progenitors. A chromosomal aberration involving Olig2 is shown to cause a form of T-cell acute lymphoblastic leukemia (T-ALL). (Ref.: Metzis, V., et al. (2018). Cell 175(4); 1105-1118).

Specificity

Other species have not been tested.

Recognizes the ~32 kDa Olig-2 protein by Western blot.

Immunogen

Recombinant mouse Olig-2

Application

Anti-Olig-2 Antibody is an antibody against Oligodendrocyte transcription factor 2 for use in IC, IH, IH(P), IP and WB.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Western Blot Analysis:
1:2,500-1:5,000 dilution of a previous lot was used in western blot on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord).

Immunoprecipitation:
1:500-1:1,000 with human oligodendroglioma and mouse transfected cell line.
Optimal working dilutions must be determined by the end user.

Immunocytochemistry:
1:500-1:1,000 dilution of a previous lot was used in immunocytochemistry on human, rat and mouse cell lines (human oligodendroglioma, rat primary neurepithelial, mouse transfected) and tissues (brain and spinal cord).

Optimal working dilutions must be determined by the end user

Target description

~32 kDa

Physical form

Ammonium Sulfate Precipitation

Format: Purified

Purified rabbit polyclonal. Liquid in 100 mM Glycine, 200 mM Tris, pH 8.0.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by immunohistochemistry on glioblastoma

Immunohistochemistry (Paraffin):
Representative lot data.
Anti-Olig2 (AB9610) staining pattern morphology in glioblastoma. Tissue was pretreated with TE Buffer, pH 9.0. Polyclonal antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB.
Optimal Staining With TE Buffer Epitope Retrieval: Glioblastoma

Analysis Note

Control
Mouse brain whole cell lysate, PC12 whole cell lysate, brain (rat) tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Introduction Methods    Human Neural Stem Cell Culture    Oligodendrocyte Differentiation and Maturation    Oligodendrocyte Progenitor Cell Characterization    In Vitro Myelination Assay Results Conc...
Christine Chen, Michael Moeller, Anna Abai, Nick Asbrock and Vi Chu1
1
MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Adhesion, Atomic absorption spectroscopy, Cell culture, Cell proliferation, Central Nervous System, Clinical, Culture media, Diseases, Gene expression, Growth factors, Hormones, Neurodegenerative Diseases, Pharmaceutical, Phase transitions, RNA immunoprecipitation, Transfection

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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