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  • AB9698 - Anti-Neuron Specific Enolase (NSE) Antibody

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AB9698 Sigma-Aldrich

Anti-Neuron Specific Enolase (NSE) Antibody

clone, Chemicon®, from chicken

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, NE-NI, Primary Antibodies
clone   polyclonal
biological source   chicken
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   rat, mouse, human
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
mfr. no.   Chemicon®
NCBI accession no.   NM_001975.2
UniProt accession no.   P09104

Description

Specificity

Recognizes Neuron Specific Enolase (NSE).

Immunogen

Synthetic peptides from human/rat/mouse Neuron Specific Enolase.

Application

Anti-Neuron Specific Enolase (NSE) Antibody is an antibody against Neuron Specific Enolase (NSE) for use in IC, IH & WB.

Immunohistochemistry: 1:50-1:100 using 2% paraformaldehyde fixed tissues.
Immunocytochemistry: 1:50-1:100 using 2% paraformaldehyde fixed cells.
Western blotting: On mouse brain extracts, blot shows a single band at 47 kDa.
Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Physical form

Affinity purified chicken IgY. Two different antibodies were combined to make this product. Liquid in PBS, pH 7.2 with 0.02% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Linkage

Replaces: AB5619

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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