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AB986 Sigma-Aldrich

Anti-β Galactosidase Antibody, bacterial

Anti-β Galactosidase Antibody, bacterial detects level of β Galactosidase & has been published & validated for use in ELISA, IP, WB, IH.

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Properties

Related Categories Antibodies, Primary Antibodies More...
trade name   Chemicon
brand family   Chemicon
format   Serum
application(s)   ELISA
  Immunoprecipitation
  Western Blotting
  Immunohistochemistry
clone   Polyclonal Antibody
epitope   bacterial
host   Rabbit
species reactivity   Vertebrates, E. coli
NCBI accession no.   NM_000404.2, NM_001079811.1
UniProt accession no.   P16279
gene symbol   EBP(10682)
  GLB1(2720)
  EC 3.2.1.23
  ELNR1
  LACTASE
  S-GAL
material size   2 mL
shipped in   wet ice
storage conditions   Maintain lyophilized material at 2-8°C for up to 12 months. After reconstitution maintain in undiluted aliquots at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Description

Physical form

Rabbit antisera prepared by delipidation and defibrination. Lyophilized. Reconstitute with 2 mL of sterile deionized water. Contains 0.02M Potassium Phosphate, 0.15M NaCl and 0.01% sodium azide after reconstitution.

Application

Immunoblotting: 1:500-1:2,000

Immunoprecipitation

Immunohistochemistry (Zhang, 2002)

ELISA: 1:2,000-1:10,000 when tested against 1 μg of immunogen by a standard sandwich ELISA.

Optimal working dilutions must be determined by the end user.

Specificity

Specific for Beta galactosidase from E coli by IEP. May cross react with Beta galactosidase from other species.

Immunogen

Beta galactosidase from E-coli.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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