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ABC129 Sigma-Aldrich

Anti-WWP2 Antibody

This Anti-WWP2 Antibody is validated for use in Western Blotting, IHC for the detection of WWP2.

Synonym: NEDD4-like E3 ubiquitin-protein ligase WWP2, WW domain-containing protein 2

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, W
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   mouse, human
shipped in   wet ice
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_080106
UniProt accession no.   Q9DBH0

Description

General description

WW2 (atrophin-1-interacting protein 2) is a member of a large family of NEDD4-like E3 ligases that transfer ubiquitin from E2 ubiquitin-conjugating enzymes to targeted protein substrates. In general, ubiquitination affects protein turnover and activity. As a result, ubiquitin-related enzymes play essential roles in many cellular processes. The WW2 E3 ubiquitin-protein ligase contains a C2 domain at the N-terminus and a HECT (E6AP-type E3 ubiquitin-protein ligase) domain at the C-terminus which interact to inhibit its catalytic activity. Previous studies have suggested that WW2 may play a role in cellular transport, transcription, and T-cell activation. It has also been shown that WW2 regulates the PTEN tumor suppressor and plays an essential role in apoptosis and survival of cancer cells. WW2-deficient mice show abnormal development of craniofacial structures.

Immunogen

GST-tagged recombinant protein corresponding to mouse WWP2.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blot Analysis: A representative lot of this antibody detected WWP2 in CGR8 ES whole cell lysate and in transiently transfected HEK293 cells (Xu, H. M. et al. (2004). J Biol Chem. 279(22):23495-23503.).

Target description

~100 kDa observed. Uncharacterized bands may be observed at ~31 kDa, ~57 kDa and ~60 kDa in some cell lysates.

Physical form

Affinity Purfied

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in mESC lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected WWP2 in 10 µg of mESC lysate (For optimal performance, a range of 1 to 2 µg/mL of this antibody is recommended in Western Blot).

Analysis Note

Control
mESC lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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