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  • ABC212 - Anti-phospho ULK1 Antibody (Thr574)

ABC212 Sigma-Aldrich

Anti-phospho ULK1 Antibody (Thr574)

clone, from rabbit, purified by affinity chromatography

Synonym: Serine/threonine-protein kinase ULK1, Serine/threonine-protein kinase Unc51.1, Unc-51-like kinase 1

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Primary Antibodies, UH-UZ
clone   polyclonal
biological source   rabbit
application(s)   inhibition assay: suitable (peptide)
  western blot: suitable
species reactivity   human
species reactivity (predicted by homology)   bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_033495
UniProt accession no.   O70405


General description

ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.


KLH-conjugated linear peptide corresponding to mouse ULK1 phosphorylated at Thr574.


Detect Autophagy-related protein;ATG1 using this rabbit polyclonal antibody, Anti-phospho ULK1 Antibody (Thr574) validated for use in western blotting & Peptide Inhibition Assay.

Western Blotting/Peptide Inhibition Analysis: 0.5 µg/mL from a representative detected phospho ULK1 in WB/PIA.

Target description

~120 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.


Evaluated by Western Blotting in untreated and Calyculin A with okadaic acid treated Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho ULK1 (Thr574) in 10 µg of Jurkat cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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