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ABC226 Sigma-Aldrich

Anti-USP7S Antibody

clone, from rabbit, purified by affinity chromatography

Synonym: Ubiquitin carboxyl-terminal hydrolase 7, Deubiquitinating enzyme 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin thioesterase 7, Ubiquitin-specific-processing protease 7

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, UH-UZ
clone   polyclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   human, human
species reactivity (predicted by homology)   mouse (based on 100% sequence homology), Xenopus (based on 100% sequence homology), rat (based on 100% sequence homology), porcine (based on 100% sequence homology)
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_003461
UniProt accession no.   Q93009
Gene Information   human ... USP7(7874)

Description

General description

The protein named Ubiquitin carboxyl-terminal hydrolase 7, or Deubiquitinating enzyme 7, Herpesvirus-associated ubiquitin-specific protease, Ubiquitin thioesterase 7, or Ubiquitin-specific-processing protease 7 and encoded by the human gene USP7/HAUSP is a ubiquitin specific hydrolase that deubiquitinates several key transcription factors and thus modulates their activity. Among the important factors are FOXO4, p53, MDM2, ERCC6, DNMT1, UHRF1, PTEN, and DAXX. Thus USP7S is involved in cell proliferation and gene expression regulation and the regulation of the stress response pathways. Interestingly recent research has linked USP7S with regulating transcription-coupled nucleotide excision repair (TC-NER) and the being important in the disease of UV-sensitive syndrome (UVSS) too. USP7S is widely expressed and up regulated in many cancers including prostate cancer.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to a region near the N-terminus of human USP7S.

Application

Anti-USP7S Antibody detects level of USP7S & has been published & validated for use in USP7S.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected USP7S in HeLa whole cell extract (Dr. Grigory Dianov, University of Oxford, United Kingdom.)

Target description

~140 kDa observed

Physical form

Affinity Purfied

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected USP7S in 10 µg in HeLa cell lysate.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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