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ABC340 Sigma-Aldrich

Anti-GOLPH1 Antibody

clone, from rabbit, purified by affinity chromatography

Synonym: Golgi phosphoprotein 1, golgi complex-associated protein 1, GOCAP1, GCP60, acyl CoA binding domain containing 3, ACBD3, PBR- and PKA-associated protein 7, Peripheral benzodiazepine receptor-associated protein PAP7, GOCAP1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, GM-GO, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   human
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
UniProt accession no.   Q9H3P7
Gene Information   human ... ACBD3(64746)

Description

General description

Golgi resident protein GCP60, or GOLPH1 is a Golgi protein critically involved in the maintenance of the Golgi structure. GOLPH1 was initially discovered through its interaction with another critical Golgi protein, giantin, also known as GOLGB1. GOLPH1 protein is widely expressed in all tissues with especially abundant expression in testis and ovary. GOLPH1 associates with giantin via its cytoplasmic C-terminal domain, and thus GOLPH1 is a peripheral Golgi membrane protein. More recently, GOLPH1 has been renamed acyl-coenzyme A binding domain containing 3 (ACBD3) and appears to be a key player in the emerging field of Golgi body-mediated signaling. GOLPH1/ACBD3 plays a central role in several cellular signaling cascades after its release from the Golgi apparatus. GOLPH1/ACBD3 is involved in cAMP-dependent steroidogenesis, apoptosis, iron homeostasis, and determination of neural progenitor cell fate and differentiation. EMD Millipore’s Anti-GOLPH1 rabbit polyclonal antibody has been tested in western blotting using human K562 cell line lysates.

Immunogen

KLH-conjugated linear peptide corresponding to human GOLPH1.

Application

Detect Golgi phosphoprotein 1 using this rabbit polyclonal antibody, Anti-GOLPH1 Antibody validated for use in western blotting.

Target description

~70 kDa observed. Uncharacterized bands may appear in some lysate(s).

Quality

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected GOLPH1 in 15 µg of K562 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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