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ABC348 Sigma-Aldrich

Anti-Bmf Antibody

clone, from rabbit, purified by affinity chromatography

Synonym: Bcl-2-modifying factor, Bmf

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, BL-BZ, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
UniProt accession no.   Q96LC9
Gene Information   human ... BMF(90427)

Description

General description

Bcl-2-modifying factor (Bmf) is a member of the Bcl-2 family containing one BCL2 homology domain 3. Members of this family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell′s actin cytoskeleton. Bmf is constitutively expressed in many tissues. There are 3 isoforms of Bmf. Isoform 1 is mainly expressed in B-lymphoid cells while isoform 2 and 3 are mainly expressed in B-CLL and normal B-cells.

Specificity

This antibody recognizes the N-terminus of Bmf.

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminus of human Bmf.

Application

Detect Bcl-2-modifying factor using this rabbit polyclonal antibody, Anti-Bmf Antibody validated for use in western blotting & IHC.

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Bmf in HeLa cells.

Target description

~25 kDa observed

Quality

Evaluated by Western Blotting in HepG2 and HEK293 cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected Bmf in 15 µg of HepG2 and HEK293 cell lysates.

Analysis Note

Control
HepG2 and HEK293 cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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