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ABC504 Sigma-Aldrich

Anti-IGF2 Antibody

This Anti-IGF2 Antibody is validated for use in Western Blotting for the detection of IGF2.

Synonym: Insulin-like growth factor 2, IGF-2, Somatomedin-A, Insulin-like growth factor 2, Insulin-like growth factor 2 Ala-25 Del, Preptin

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, I-IG, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   human
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_000603
UniProt accession no.   P01344
Gene Information   human ... IGF2(3481)

Description

General description

Human Insulin-like Growth Factor 2 (IGF2) contains 67 amino acids and shares similar structural features with IGF1, including a 62% sequence homology. In human plasma, IGF1 and IGF2 are associated with IGF-binding proteins that transport the polypeptides and partially regulate their actions in vivo. In addition to the insulin receptor, IGF2 binds to two forms of IGF receptors, both of which are widely distributed in different tissues and cultured cells. IGF2 is mitogenic for a variety of cultured cells, including mouse 3T3 cells,12 normal rat kidney cells, human or chicken fibroblasts and MCF-7 human breast carcinoma cells.

Immunogen

Epitope: Insulin-like growth factor 2 mature chain

KLH-conjugated linear peptide corresponding to the human Insulin-like growth factor 2 mature chain.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Target description

~24 kDa observed. This antibody detects all 3 isoforms of IGF2 (20-26 kDa), and may run higher than expected due to glycosylation.

Physical form

Affinity Purfied

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in human placenta tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected IGF2 in 10 µg of human placenta tissue lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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