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  • ABD69 - Anti-Nestin, Human Antibody

ABD69 Sigma-Aldrich

Anti-Nestin, Human Antibody

Detect Nestin, Human using this Anti-Nestin, Human Antibody validated for use in Western Blotting, ICC & IHC.

Synonym: Nestin

  •  eCl@ss 32160702



Related Categories Antibodies, Primary Antibodies More...
packaging   50 µL (ABD69)
format   Serum
molecular weight   The anti-Nestin antibody has an observed MW at 220kDA. Antibody may see 260kDa band in some cells and/or treatments
application(s)   Immunocytochemistry
  Western Blotting
clone   Polyclonal Antibody
host   Rabbit
species reactivity   Human
Species Reactivity Notes   Demonstrated to react with Human. Antibody is not reactive with rat and mouse Nestin protein.
NCBI accession no.   NP_006608
UniProt accession no.   P48681
gene symbol   FLJ21841
shipped in   wet ice
Quality Assurance   Evaluated by Immunocytochemistry in human U251 glioblastoma cells. Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human U251 glioblastoma cells.


Analysis Note

U251 glioblastoma cells.


Evaluated by Immunocytochemistry in human WI38 cells.
Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human WI38 cells.
Evaluated by Immunohistochemistry in human glioblastoma tissue.
Immunocytochemsitry Analysis: A 1:2,000 dilution of this antibody detected Nestin in human glioblastoma tissue.
Evaluated by Western Blot in human U251 glioblastoma cells. Western Blot Analysis: A 1:25,000 dilution of this antibody has been shown to detected Nestin in human U251 glioblastoma cells.

General description

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.


GST-tagged recombinant protein corresponding to human Nestin.


This antibody recognizes human Nestin. Antibody is not reactive with rat and mouse Nestin protein.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis


Making Headway
Protocols & Articles


Neural Stem Cell Culture Protocols

Introduction Methods     Isolation of Neural Stem Cells         Isolation of NSCs from Neural Tissue         Differentiation of Human iPSCs into NSCs         Characterization of Neural Stem Cells    ...
Keywords: AGE, Adhesion, Apoptosis, Cell culture, Central Nervous System, Cryopreservation, Culture media, Diseases, Growth factors, High performance liquid chromatography, Phase transitions, Phosphorylations, Purification

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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